Peter Parker, the amazing Spider-Man, has made an astonishing discovery -- he has a sister! Worse, a manipulative super-villain known as the Gentleman, who had a hand in the deaths of Peter Parker's parents, has somehow brainwashed her into becoming the deadly super-villain known as Pity! Together with Doctor Octopus, Electro, the Vulture, and Mysterio, they've formed the newest incarnation of the super-villain group dedicated to destroy Spider-Man, the Sinister Six. The Gentleman's plan to destroy Spider-Man is about to reach it's awful conclusion!